diff --git a/changelog_unreleased/javascript/20043.md b/changelog_unreleased/javascript/20043.md
new file mode 100644
index 000000000..7b674b648
--- /dev/null
+++ b/changelog_unreleased/javascript/20043.md
@@ -0,0 +1,30 @@
+#### Preserve comment order in test calls (#20043 by @lllleolin-max)
+
+<!-- prettier-ignore -->
+```jsx
+// Input
+test("x", () => {
+  run();
+}, // first
+// second
+// third
+60000);
+
+// Prettier stable
+test("x", () => {
+  run();
+}, // second // first
+// third
+60000);
+
+// Prettier main
+test(
+  "x",
+  () => {
+    run();
+  }, // first
+  // second
+  // third
+  60000,
+);
+```
diff --git a/src/language-js/utilities/test-libraries.js b/src/language-js/utilities/test-libraries.js
index f8207423f..f1788d58c 100644
--- a/src/language-js/utilities/test-libraries.js
+++ b/src/language-js/utilities/test-libraries.js
@@ -1,4 +1,5 @@
 import { getCallArguments } from "./call-arguments.js";
+import { hasComment } from "./comments.js";
 import { getFunctionParameters } from "./function-parameters.js";
 import { isNodeMatches } from "./is-node-matches.js";
 import {
@@ -104,6 +105,10 @@ function isTestCall(node, parent) {
 
   const args = getCallArguments(node);
 
+  if (args.some((arg) => hasComment(arg))) {
+    return false;
+  }
+
   if (args.length === 1) {
     if (isAngularTestWrapper(node) && isTestCall(parent)) {
       return isFunctionOrArrowExpression(args[0]);
diff --git a/tests/format/js/test-declarations/__snapshots__/format.test.js.snap b/tests/format/js/test-declarations/__snapshots__/format.test.js.snap
index 581d64d24..9fa6c0c81 100644
--- a/tests/format/js/test-declarations/__snapshots__/format.test.js.snap
+++ b/tests/format/js/test-declarations/__snapshots__/format.test.js.snap
@@ -534,6 +534,151 @@ function x() {
 ================================================================================
 `;
 
+exports[`comments.js - {"arrowParens":"avoid"} format 1`] = `
+====================================options=====================================
+arrowParens: "avoid"
+parsers: ["babel", "flow", "typescript"]
+                                                      printWidth: 80 (default) |
+=====================================input======================================
+test("comments after callback", () => {
+  run();
+}, // first
+// second
+// third
+60000);
+
+it("comments before timeout", function (done) {
+  done();
+},
+// first
+// second
+60000);
+
+describe.only("comments after callback", () => {
+  run();
+}, // first
+// second
+60000);
+
+test("comments before callback", // first
+// second
+() => {
+  run();
+});
+
+=====================================output=====================================
+test(
+  "comments after callback",
+  () => {
+    run();
+  }, // first
+  // second
+  // third
+  60000,
+);
+
+it(
+  "comments before timeout",
+  function (done) {
+    done();
+  },
+  // first
+  // second
+  60000,
+);
+
+describe.only(
+  "comments after callback",
+  () => {
+    run();
+  }, // first
+  // second
+  60000,
+);
+
+test(
+  "comments before callback", // first
+  // second
+  () => {
+    run();
+  },
+);
+
+================================================================================
+`;
+
+exports[`comments.js format 1`] = `
+====================================options=====================================
+parsers: ["babel", "flow", "typescript"]
+                                                      printWidth: 80 (default) |
+=====================================input======================================
+test("comments after callback", () => {
+  run();
+}, // first
+// second
+// third
+60000);
+
+it("comments before timeout", function (done) {
+  done();
+},
+// first
+// second
+60000);
+
+describe.only("comments after callback", () => {
+  run();
+}, // first
+// second
+60000);
+
+test("comments before callback", // first
+// second
+() => {
+  run();
+});
+
+=====================================output=====================================
+test(
+  "comments after callback",
+  () => {
+    run();
+  }, // first
+  // second
+  // third
+  60000,
+);
+
+it(
+  "comments before timeout",
+  function (done) {
+    done();
+  },
+  // first
+  // second
+  60000,
+);
+
+describe.only(
+  "comments after callback",
+  () => {
+    run();
+  }, // first
+  // second
+  60000,
+);
+
+test(
+  "comments before callback", // first
+  // second
+  () => {
+    run();
+  },
+);
+
+================================================================================
+`;
+
 exports[`jest-each.js - {"arrowParens":"avoid"} format 1`] = `
 ====================================options=====================================
 arrowParens: "avoid"
diff --git a/tests/format/js/test-declarations/comments.js b/tests/format/js/test-declarations/comments.js
new file mode 100644
index 000000000..1b439f0ca
--- /dev/null
+++ b/tests/format/js/test-declarations/comments.js
@@ -0,0 +1,25 @@
+test("comments after callback", () => {
+  run();
+}, // first
+// second
+// third
+60000);
+
+it("comments before timeout", function (done) {
+  done();
+},
+// first
+// second
+60000);
+
+describe.only("comments after callback", () => {
+  run();
+}, // first
+// second
+60000);
+
+test("comments before callback", // first
+// second
+() => {
+  run();
+});